Detection of Alkaloids

The alkaloids are heterocyclic compounds, generally derived from amino acids, containing one or more nitrogen atoms in its structure, which are part of the largest group of plant secondary metabolites. They are soluble both in polar and non-polar solvents, and characterized by a powerful physiological activity. On the one hand, due to their special activities, alkaloids are widely used in fields such as healthcare, cosmetics, food, and pesticides. On the other hand, some alkaloids have toxic or strong physiological effects on the living organism. Therefore, the content of alkaloids needs to be strictly monitored in various fields.

Our Detection Methods

Our alkaloid detection methods include but are not limited to the following.

Thin Layer Chromatography (TLC)

TLC is a method of separation, or identification of a mixture of components by using finely divided adsorbent solid/ liquid over a glass plate, and liquid as a mobile phase. TLC can be used to help determine the number of alkaloids in a mixture, the identity of alkaloids, and the purity of an alkaloid.

High Performance Liquid Chromatography (HPLC)

HPLC is an analytical technique used to separate, identify, or quantify each component in a mixture. HPLC is characterized by high separation efficiency, high sensitivity, low sample usage, automated online detection, low cost, and various modes. HPLC can be used in combination with UV detector, diode array detector, fluorescence detector, evaporative light detector, and electrochemical detector respectively, all of which can detect the content of alkaloids, and the results obtained are highly accurate.

Gas Chromatograph-Mass Spectrometer (GC-MS)

GC-MS is a hyphenated analytical technique that combines the separation properties of GC with the detection feature of MS to identify different substances within a sample. GC-MS is suitable for some alkaloids that are volatile and do not decompose when exposed to heat, such as matrine, ephedrine, arecoline, etc.

Ultraviolet-Visible (UV-Vis) Spectroscopy

UV-Vis Spectroscopy is an analytical method that can measure the analyte quantity depending on the amount of light received by the analyte. Alkaloids have certain optical activity, and their content can be determined by UV-Vis Spectroscopy.

Acid Dye Colorimetry

Acid dye colorimetry mainly uses chemical reactions between alkaloids and acid dyes to produce ion pairs. The ion pairs are quantitatively dissolved in certain organic solvents, and finally the colorimetric determination can be performed. This method is generally used for the determination of total alkaloid content.

Our Services

Our alkaloid detection services include but are not limited to the following.

Our Advantages

• Highly Reliable: Our researchers have many years of experience to ensure the standardization of experimental operations and procedures.
• Competitive Prices: We provide customers with the most comprehensive services at the most favorable price, and help customers save funds reasonably.
• Fast Turnaround Time: We will provide detection data and feedback to our customers in a short time to meet the needs quickly.
• Advanced Platform: We have advanced technology platforms to help you obtain reliable detection results.

Service Process

Alfa Chemistry provides professional alkaloid detection services to meet customer needs. If you want to know more about the service, please contact us immediately. Alfa Chemistry is always your project partner.